Genova Bio UV/Visible Diode Array Scanning Spectrophotometer (w/ DMV-BioCell)
The Genova Bio is a simple, low-cost, easy-to-use UV/visible spectrophotometer; dedicated to life science applications. As well as the dedicated life science measurement modes this instrument can also be used as a standard spectrophotometer with measurement modes for photometrics, concentration, spectrum scanning, quantitation, and kinetics. The fast, responsive color touchscreen and pre-programmed methods for the measurement of nucleic acid concentration and purity, protein assays, and cell density, make this spectrophotometer the ideal addition to any laboratory. The Genova Bio utilizes diode array technology to scan the entire wavelength range (198 to 800nm) in less than 3 seconds and the narrow spectral bandwidth of 3nm, makes it ideal to identify peaks for determining nucleic acid purity. The 1024-element diode array detector coupled with a flash xenon lamp results in a long-life, robust spectrophotometer.
The Nucleic Acids measurement mode can be used to quantify the concentration and purity of dsDNA, ssDNA, RNA, and oligonucleotides; using wavelengths recorded at 260, 280, and 230nm, with an optional correction at 320nm. The concentration is calculated along with the corresponding ratios. At the touch of a button, it is easy to check the purity of the nucleic acids. This is done by identifying distorted peaks in the purity scan between 200 and 350nm. This is especially useful for RNA samples where impurities may be present at 230nm, but cannot be detected using the 260/280nm ratio measurement.
The Proteins measurement mode can be used to calculate protein concentration by creating standard curves from protein assay kits. With pre-programmed methods for measuring Bradford, Lowry, Biuret and Bicinchoninic Acid (BCA) assays; up to 6 standards can be measured with 3 replicates of each standard to minimize any dilution errors. The Proteins measurement mode is also pre-programmed with the Direct UV and Warburg-Christian methods to determine protein purity.
This Genova Bio UV/Visible scanning spectrophotometer comes with the DMV-BioCell cuvette which offers high accuracy and reproducibility for direct, low-volume measurements of nucleic acids or proteins without the need for a dedicated system. The optical path can be validated to a high degree of accuracy with the included DNACON stable certified reference materials for 260/280 ratio validation.